Single Cell Analysis

Project Description

Bulk analysis of a heterogeneous population of cells can be misleading due to averaging of the signals. In contrast, single cell analysis of individual cells can yield critical information which otherwise may not be detected, especially if these cells are rare. One example is that of the heterogeneous population of circulating tumor cells where they can be epithelial, mesenchymal, EMT or cancer stem-like. Here, we develop a variety of microfluidic platforms including droplet based microfluidics to perform analysis of individual circulating tumor cells. Our aim is to probe their intracellular heterogeneity and profile their mutation signatures. These patient-derived information can potentially be useful in determining the right treatment for the right patient at the right time, thus leading to precision or personalized medicine.

Selected Publications

1. Addressing cellular heterogeneity in tumor and circulation for refined prognostication

Lim, S B, et al., PNAS, 2019. [in press]

2. Monitoring of cancer patients via next-generation sequencing of patient-derived circulating tumor cells and tumor DNA

Onidani, K, et al., Cancer Science, 0, 1-10, 2019.Article

3. Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells

Abouleila, Y, et al., Cancer Science, 110, 697-706, 2019.Article

4. Microfluidic enrichment for the single cell analysis of circulating tumor cells

Yeo, T, et al., Scientific Reports, 6, 22076, 2016.Article

5. Jetting microfluidics with size-sorting capability for single-cell protease detection

Jing, T Y, et al., Biosensors & Bioelectronics, 66, 19–23, 2015.Abstract

6. Single cell kinase signaling assay using pinched flow coupled droplet microfluidics

Ramji, R, et al., Biomicrofluidics, 8, 034104, 2014.Article

7. Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns

Lin, L Y, et al., Lab on a Chip, 13, 714-721, 2013.Abstract

8. Microfluidic single mammalian cell lysis in pico-litre droplets

Ramji, R, et al., Journal of Biosensors & Bioelectronics, S:12, 2013.Article

About the MechanoBioEngineering Laboratory

We seek to address important scientific and biomedical problems using interdisciplinary approaches, develop innovative solutions and translate them for biological and healthcare applications. Our focused areas include mechanobiology of human diseases and development of mechanobiologically inspired platforms in microfluidics and wearable microdevices to better detect, diagnose and treat diseases. We have won numerous research awards and honors and have also spun out several startup companies to translate technologies from our lab to bedside and market. We are affiliated with the iHealthtech, Mechanobiology Institute, Centre for Advanced 2D Materials and SMART.

Contact Us
MechBioEng Lab

T-Lab, #08-03, 5A Engineering Drive 1
Dept of Biomedical Engineering
NUS, Singapore 117411
Tel: (65) 6516 6564
Fax: (65) 6773 2205